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GlossWell #360 Type Anti-Viral

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12/28, 2020 : Inactivation of COVID-19 has been demonstrated by a third-party organization.

December.28,2020 : QTEC (Kobe Testing Center, Japan Textile Products Quality Technology Center) has demonstrated that COVID-19 can be inactivated.
The antiviral and antimicrobial compound (special quaternary ammonium salt) contained in GlossWell #360 Type Anti-Viral has been proven to inactivate COVID-19. The special antiviral and antimicrobial coating of GlossWell #360 Type Anti-Viral has a strong adhesion to the surface on which it is applied, and it also reduces viruses and bacteria on the coating within a short period of time (99.9% reduction after 10 minutes). We hope that COVID-19 will help reduce the risk of contact infections by creating an antiviral environment through coating in areas where many people come into contact with it or where droplets must be avoided.

[ Antiviral test using COVID-19 ]
◯ Client: Presence, Inc.
◯ Testing site: QTEC (Kobe Testing Center, Japan Textile Products Quality Technology Center)
◯ Date of the test result report: December 28, 2020
◯ Test method: ISO 21702
◯ Test virus: SARS-CoV-2 / NIID isolate; JPN/TY/WK-521 (distributed by the National Institute of Infectious Diseases)
◯ Sample : GlossWell #360 Type Anti-Viral ( unprocessed product )
◯ Test sample : GlossWell #360 Type Anti-Viral ( processed product )
◯ Test result: Antiviral activity value after 24 hours of exposure ≧3.2 (▶︎ Numerical explanation)
▶︎ Numerical explanation : Antiviral activity value ≧3.2 means that the antiviral activity value is 99.9% or more than 1/1000.
※ The antiviral activity value that is considered acceptable by ISO 21072 is ≧2.0, so the results of this test far exceed the acceptable value.

Paint manufacturer’s statement
Since GlossWell #240 / #750 Type Anti-Viral has the same composition / component structure as GlossWell #360 Type Anti-Viral and uses the same antiviral and antibacterial compounds (special quaternary ammonium salts), chemical considerations lead us to conclude that it is as effective against COVID-19 as GlossWell #360 Type Anti-Viral.

GlossWell #360 Type Anti-Viral

Has antiviral function.
One and only very special glass coat.
Extremely long lasting it will be make a strongest killer coating.
It could be easily apply to the various construction surface.
We Introducing new options to protect for your life.

GlossWell # 360: Type Anti-Viral is based on an inorganic (real glass) glass coating agent, which allows various viruses and bacteria / fungi to survive and proliferate. It is the latest special agent with anti-virus / anti-bacterial / anti-fungal function, which has special compounds derived from ammonium to be suppressed. Unlike conventional spray-type liquid and anti-microbial products such as wet tissue type, it is an agent, so it has a very long lasting performance on the construction surface without concerned with any type of shape or condition of the construction surface. It is possible to make a form for natural killer antibacterial coating. GlossWell #360: The cured coating formed by Type Anti-Viral has excellent performance for water resistance and against oil components, weather resistance when used outdoors, and various resistance to like a ethanol’s such as chemical resistance is also confirmed.
This products made in Japan.

Inactivation of COVID-19. Also approved by the METI of Japan approves

Announced by METI of Japan
On May 29, 2020, the National Institute of Technology and Evaluation (NITE) and the Ministry of Economy, Trade and Industry (METI) announced quaternary ammonium salts: benzalkonium chloride (approved) and benzethonium chloride/dialkyl dimethyl ammonium chloride (both additionally approved on May 28, 2020) as effective surfactants for COVID-19. (both to be additionally approved on May 28, 2020) were announced at the same time.

Quaternary ammonium salts approved as effective against COVID-19
◯ Benzalkonium chloride
◯ Benzethonium chloride
◯ Dialkyldimethylammonium chloride

Special quaternary ammonium salt is used as an anti-bacterial ingredient.

The special anti-bacterial compound in GlossWell #360 Type Anti-Viral is a special quaternary ammonium salt based on existing quaternary ammonium salts (benzalkonium chloride / benzethonium chloride / dialkyldimethylammonium chloride).

Features of GlossWell # 360 Type Anti-Viral

GlossWell # 360: Type Anti-Viral is a moisture-curable inorganic paint based on SI-O (siloxane) bonds. Can be applying any various type of material with various metals / resin base materials / plastic base materials / glass / wood / concrete, etc., by reacting with water vapor in the air, performance characteristics and sustainability in the construction aspect. Form a hard killer coating with the most effective anti-virus and anti-bacterial functions. The coating is also excellent in super water resistance, super release properties for various oil components, weather resistance when used outdoors, and various chemical resistance properties such as ethanol.

GlossWell # 360: Type Anti-Viral realizes “antibacterial environment” by simple painting method.
It is possible to freely applying to hard antibacterial coating excellent in sustaining effect without regard to the any type of the construction surface.
It is a special functional agent based on inorganic glass (real glass) that exists in the natural world, unlike organic glass coats that are artificially generated, individual properties of various substrates effectively cured killer coating that exhibits maximum and longest adhesion performance.

Inactivate COVID-19.
・2020.12.28 : Inactivation of COVID-19 was demonstrated in an antiviral test conducted by the Virus Research Institute of Japan (QTEC).
・Special quaternary ammonium salts are used.

Anti-viral and anti-bacterial properties.
・Please refer to the list of various test data :

Inhibits the growth of microorganisms in a short time.
・After 10 minutes, the population of microorganisms in the environment is reduced by 99.9%.

Excellent super water and oil repellent performance.
・It can easily remove fingerprints, various oily substances, blood and body fluids, dirt, etc.

Can be applied by simple painting.
・Cured coating film with excellent adhesion is formed.
・Can be applied to various base materials with only one coat.
・Please check the adhesion in advance.

Excellent in solvent resistance and electrical insulation.
・The coating film is non-flammable.

Transparent and colorless coating after curing.
Forms a smooth final coating with excellent smoothness.

GlossWell # 360 Type ANTI-VIRAL mechanism

GlossWell #360 Type Anti-Viral is a one-component special ceramic coating (called a binder) with strong adhesion to a variety of materials and an array of special quaternary ammonium salts (proven to be effective against new coronaviruses) as anti-viral and anti-bacterial ingredients.

GlossWell #360 Type Anti-Viral / Structure of LIGHT

 The following figure illustrates the mechanism by which the special quaternary ammonium salt contained in GlossWell #360 Type Anti-Viral inhibits and suppresses the growth of COVID-19 and other viruses with envelope membranes (such as influenza A viruses). Quaternary ammonium salts, which are also surfactants, disrupt the lipid bilayers and membrane proteins of the envelope of COVID-19. The virus dies when the envelope membrane is destroyed.

GlossWell #360 Type Anti-Viral : A mechanism used to inhibit and suppress the growth of viruses.

Places where you want

Please try to be applied to general households or public facilities, etc., “anything that can be touched by an unspecified number of people”.
It can be applied to various metals / resin base materials / plastic base materials / glass / concrete / wood etc.
Various door knobs and handrails / Hanging leather and handrails for public transportation / Car doors:

Handles for inner and outer parts / Tables and chairs in general houses and stores, operation buttons for counters / elevators, etc. / Baskets and carts in supermarkets / toilets Peripheral equipment: Toilet seats, etc./ Shared lockers / Water supply peripheral equipment: Faucet and hand washing, etc./ Telephones / Copy machine operation buttons / Floors and corridors of general houses, stores, factories, etc./ General gym equipment / tools / Various game equipment peripherals Operation buttons / Children’s toys and playground equipment, etc.

Construction method

The installation method is extremely simple with one-pack type paint.
・A neutral detergent with excellent oil removal function to remove oil and dirt on the construction surface.
・GlossWell # 360 Type Anti-Viral should be used undiluted without dilution.
・Apply to the construction surface with a brush / brush / microfiber / spray gun / roller / dipping etc.

Estimated range of construction
・GlossWell # 360 Type Anti-Viral x 1ℓ, about 11m2 can be constructed.
・The amount of GlossWell # 360 Type Anti-Viral used for A4 size area should be about 1ml.
・The amount of GlossWell # 360 Type Anti-Viral used when using a non-saturated base / paint brush.
・GlossWell # 360 Type Anti-Viral is completed in a thin film / no thick coating is required.
・The appropriate coating thickness (3 to 8μm) is equivalent to one or two food wrap films.

Apply paint to the construction surface using
・brush, microfiber, spray gun, roller, etc.
・Touch dry time: 30 ~ 40 minutes (You can touch the painted surface with your finger after 30 ~ 40 minutes from painting).
・Drying time at room temperature: 24 hours / However, it takes about 5 days to completely cure the coating.
・In the case of forced drying: 70-80 ° C x 30 minutes / Complete curing is about 3 days at room temperature after forced drying.

Film thickness3~8μm
Application amount50~100g/㎡
Paint viscosity9-10 seconds / HIS NK-2
Recommended spray gun diameter1.3~1.5㎜φ
Spray gun air pressure0.3~0.4MPa(3~4kgf/c㎡)
Touch dry30-40 minutes
Room temperature drying24 hours, full curing about 5 days
Forced drying70-80 ° C x 30 minutes / Complete curing is forcible drying and then drying at room temperature for 5 days

Advantages of special agent with antiviral and bacterial functions

Advantages of special agent with antiviral and bacterial functions.For example, there are many products that have an antibacterial at the manufacturing stage, such as underwear and clothing, bedding, eyeglass bleeding, hanging leather as in the trains, cutting boards, shoes, etc. These are not going to be straightforward when it comes to antibacterial measures against “things that exist in / things that cannot move.” It is possible to “disinfect” things with ethanol but when you try to create an “antibacterial environment”, how would it be? Can you really do this in the world? The solution was developed a some paint agent with antibacterial products (antivirus / antibacterial / antifungal). But it is a liquid , it is just to painting there, so “things that are already there / things that can not move”, and even if it is a complicated shape, furthermore you can freely create an antibacterial environment regardless of the products size with construction surface.

GlossWell # 360: Type Anti-Viral was developed a special agent based on such an idea, with excellent antibacterial performance (antivirus / antibacterial / antifungal), super water resistance and super release oil components performance. In addition, inorganic glass that exists in the natural world is the base of the entire coating material, and its properties make use of its strong adhesion to various substrates (siloxane bond). The coating after complete curing is clear base and transparent. The feels also very smooth, and there is no discomfort even if existing items are covered with an antibacterial environment as veil. Since this agent is completed with only one coat, the installation method is extremely simple. Of course anyone can do this by your self.

Export of GlossWell Type Anti-Viral from Japan
Please let more people know about this page! 
GlossWell #360 / #240 / #750 Type Anti-Viral is a paint. Since paint is classified as a hazardous material internationally, the cost of exporting it from Japan by air or sea is very high, and it is very difficult for an individual to handle the payment of freight and customs clearance for importation.

In this page, we present a special paint containing an antimicrobial compound that inactivates COVID-19. Grosswell #360 / #240 / #750 Type Anti-Viral agents to companies, trading companies and government officials in your country. We are publishing all the evidence needed to prove its effectiveness against COVID-19. All of this evidence has been tested and obtained by Japanese public institutions.

GlossWell #360 / #240 / #750 Type Anti-Viral forms a very tough and special coating with anti-bacterial properties. The coating will adhere to the surface for a long time. If a similar special coating does not exist in your country, please inform any person or entity that can import GlossWell #360 / #240 / #750 Type Anti-Viral or any government official of the existence of this page!

Antiviral test : COVID-19

◯ Sample submission: November 4, 2020 / Response date: December 28, 2020
◯ Client: Presence, Inc.
◯ Test item: Antiviral activity test
◯ Test method: ISO21702 / Measurement of antiviral activity on plastics and other non-porous surfaces
◯ Test organization: QTEC, Japan Textile Products Quality Technology Center, Kobe Testing Center, Microorganism Testing Laboratory

【 Test Summary 】
◯ Test virus: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) / NIID isolate:
JPN/TY!WK-521 (distributed by the National Institute of Infectious Diseases)
・Host cell:VeroE6/TMPRSS2 JCRB1819
・Cytoplasmic drop : Dulbecco’s modified Eagle’s medium (low-glucose) ; DMEM
(SIGMA, Cat#D6046) Minimum Essential Medium Eagle ; EMEM (SIGMA, Cat#-M4655)
・Fetal Bovine Serum : Fetal Bovine Serum (FBS) (SIGMA, Cat#l 73012)
・Sealing film: Polyethylene film
・Control sample : GlossWell #360 Type Anti-Viral (Unprocessed products)
・Test sample : GlossWell #360 Type Anti-Viral (Processed products)
・Purification of test specimen: Not performed
・Inoculation of test virus suspension: 0.4ml
・Test condition: Action temperature: 25°C
・Test condition: Action time: 24 hours (The virus infection titer was also measured immediately after inoculation for the control sample.
・Washing solution: SCDLP diluted 10 times in DMEM containing 2% FBS
・Infection titer measurement method: Plaque measurement method

【 Test Operation 】
1 ) Main Test :
1. Infect host cells with the virus, add EMEM and incubate at 37°C for a predetermined period of time, then centrifuge at 4°C, l,000xg for 15 minutes, and the supernatant is used as the test virus suspension.
2. Dilute the virus suspension obtained in 1. 10-fold with sterile distilled water to 1 ~sx101 PFU/mL, and use this as the test virus suspension.
3. Place each specimen (50 mm x 50 mm) on the bottom of a sterile Petri dish with the processed side up, and inoculate with 0.4 ml of the test virus suspension.
4. Cover the petri dish with an adhesive film (40 mm x 40 mm) and press down lightly so that the test virus suspension is spread over the entire film.
5. Cover the petri dish with the lid.
6. After dying at 25°C for 24 hours at 90%RH or higher, add 10 mL of washing-up liquid to each test sample.
7. Scrub the surface of each test specimen and the dense cling film to wash out the virus.
8. Dilute the washout solution 10-fold using DMEM containing 2% FBS.
9. Determine the viral infection titer by the plaque measurement method.

2 ) Host cell verification test :
2 )-1 Cytotoxicity Confirmation
1. Add l0mL of washing-up liquid to each test sample, and perform the washing-up procedure as in the main test.
2. Make a 10-fold dilution of the washout solution using DMEM containing 2% FBS.
3. Stain the cells as in the plaque measurement method to check for cytotoxicity.

2 )-2 Susceptibility testing of cells to viruses
1. Add 10mL of washing-up liquid to each test sample and perform the washing-up procedure as in the main test.
2. Dilute the washout solution 10-fold using DMEM containing 2% FBS.
3. Take 5 mL of the solution in 2. above into a sterile test tube.
4. Prepare a test virus suspension at 4~6×104 PFU/ml using EMEM, and add 0.05 ml of the suspension to the washout solution in 2.
5. Allow to stand at 25°C for 30 minutes.
6. Measure the virus infection titer by the plaque measurement method and confirm the susceptibility of the cells to the virus by measuring the virus infection titer per ml of washout solution.

Test Results
1 ) Main Test :
・Test virus: SARS-CoV-2 NIID isolate; JPN/TY!WK-521 (distributed by the National Institute of Infectious Diseases)
・Test virus suspension concentration: 1.2 x 107 PFU/ml

 SpecimenVirus infection titer (PFU/cm2) (Note 2)
Logarithmic value for normal use
Virus infection titer (PFU/cm2) (Note 2)
log-average normalized value
Antiviral activity value 【R】
(Note 2)
GlossWell #360 Type Anti-Viral (Unprocessed) (Note 1)
Immediately after inoculation【U0
n1 / 5.525.53
same as aboven2 / 5.525.53
same as aboven3 / 5.555.53
GlossWell #360 Type Anti-Viral (Unprocessed product) (Note 1) After leaving for 24 hours【Utn1 / 5.045.04
same as aboven2 / 5.035.04
same as aboven3 / 5.065.04
GlossWell #360 Type Anti-Viral (Processed product)
After leaving for 24 hours
【At
n1  <1.80 <1.80
same as aboven2  <1.80 <1.80
same as aboven3  <1.80 <1.80 ≧3.2 [ Numeric Description ]

The antiviral activity value ≧3.2 means that the antiviral activity value after 24 hours is 99.9% or more than 1/1000.
※ The antiviral activity value that is considered acceptable by ISO 21072 is ≧2.0, so the results of this test far exceed the acceptable value.

(Note 1) : GlossWell #360 Type Anti-Viral (unprocessed) (provided by the client) was used as a control sample.
(Note 2) PFU : plaque forming units.
(Note 3) Antiviral activity value R= Ut -At

2 ) Host cell verification test
・Test virus: SARS-CoV-2 NIID isolate; JPN/TY/WK-521 (distributed by the National Institute of Infectious Diseases)
・Test virus suspension concentration: 4.9 x 104PFU/ml

Specimen2 ) -1 Presence of cytotoxicity2 ) -2 Confirmation of cell susceptibility to the virus.
Virus infection titer (PFU/ml) (Note 2) Logarithmic mean value for normal use
Determination of test validity
GlossWell #360 Type Anti-Viral (Unprocessed) (Note 1)non-【 Su 】  2.68establishment
GlossWell #360 Type Anti-Viral (Processed product)non-【 Su 】  2.69establishment
Negative control (Note 4)non-【 Sn 】  2.67

(Note 4) A solution of SCDLPs diluted 10-fold in DMEM containing 2% FBS was used as a negative control.

[ Conditions for completion of the test ]
2-1 ) Cytotoxicity: None
2-2 ) Confirmation of cell susceptibility to the virus: | Sn – Su | ≤ 0.5 and | Sn – S1 | ≤ 0.5

【 Reference Information 】
◯ Real-time RT-PCR measurement of virus suspensions subjected to this test.
・Test virus: SARS-CoV-2NIID isolate; JPN/TY/WK.-521 (distributed by the National Institute of Infectious Diseases)
・Virus suspension wastiness: >lOSPFU/ml
・Real-time PCR equipment: Thermal Cycler Dice Real Time SysteM 3 (TaKaRa)
・Detection Kit: SARS-CoV-2Detection Kit -Nl set- (Code NCV-301; Lot# 038200)
(TOYOBO CO.,LTD. Biotech support Department)

◯ Measurement results
The amplification of viral RNA was confirmed by the real-time RT-PCR measurement results (Fig. l).

Fig.I. Real-time RT-PCR measurement results of virus suspension

◯ Graph: red line (102-fold dilution of viral suspension flooding in PBS)
◯ Graph: Blue line (103-fold dilution of virus suspension wastage in PBS)
◯ Graph: black line (104-fold dilution of virus suspension wastage in PBS)
◯ Graph: pink line (Negative control; EMEM)

Testing organization: Microbiological Testing Laboratory, Kobe Testing Center, Japan Textile Quality Technology Center

Antiviral test: Influenza A virus (with an envelope membrane)

◯ Specimen: April 2, 2020 / Reply date: June 5, 2020
◯ Test item: Antiviral test
◯ Test details: Evaluate the antiviral properties of polycarbonate sheets
◯ Test method: ISO21702 / Measurement of antiviral activity on plastics and other non-porous surfaces
◯ Testing organization: Microbiology Laboratory, Kobe Testing Center, Japan Textile Quality Technology Center

[ Test Summary ]
・Study virus: Influenza A virus (H3N2) A/Hong Kong/8/68; TC adapted ATCC VR-1679
・Host cells : MDCK cells (canine kidney-derived cells)
・Test sample
(1) GlossWell #360 Type Anti-Viral / Polycarbonate sheet (unprocessed) / control: Sample submitted by the client
(2) GlossWell #360 Type Anti-Viral / Polycarbonate Board (Processed)
・SCDLP medium
・Leaving conditions : Leaving temperature 25°C
・Time of storage 24 hours :  (1) GlossWell #360 Type Anti-Viral polycarbonate sheet (unprocessed) was measured immediately after leaving the product for 24 hours.
・Sample size : 5cm x 5cm
・Adhesion film: Polyethylene (4cm x 4cm)
・Inoculation volume of the test virus suspension: 0.4 mL
・Cleanliness of the test specimens: Not conducted.

[ Test Operation: Main Test ]
1. Infect host cells with the virus and culture them, and remove the remaining cells by centrifugation to make a virus suspension.
2. dilute the virus suspension 10-fold with sterile distilled water to a concentration of 1-5X10∧7 PFU/mL, and treat as a test virus suspension.
3. Place each specimen on the bottom of a sterile petri dish with the processed side up, and inoculate it with 0.4 mL of the test virus suspension. 
4. Cover the Petri dish with adhesive film and press down lightly so that the test virus suspension is spread over the entire film.
5. Cover the petri dish with the lid.
6. Add 10 mL of washout solution to each test specimen after 24 hours at 25°C. 
7. Scrape the surface of each test specimen and the contact film to remove the virus.
8. Measure the viral titer by the plaque assay.

[ Host cell verification test ]
2 )-1 Cytotoxicity confirmation test
1 ) Add 10mL of washout solution to each test specimen, and perform the washout procedure as in this test. 2) Stain cells as in the plaque assay, and confirm the presence of cytotoxicity.
2 ) Stain cells in the same way as the plaque assay and confirm the presence or absence of cytotoxicity.
2 )-2 Confirmation of cell susceptibility to viruses
1. Add 10mL of washing-up liquid to each test specimen, and perform washing-up operation as in this test. 
2. 5 mL of the above washout solution is placed in a sterile test tube.
3. Prepare 4-6 X 10 ∧ 4 PFU/mL test virus suspension, and add 0.05 mL of the suspension to the washout solution (2).
4. let it stand at 25°C for 30 minutes.
5. confirm the susceptibility of the cells to the virus by measuring the viral infection titer by the plaque assay.

[ Test method ]
1 ) Main test
・Test virus: Influenza A virus (H3N2), A/Hong Kong/8/68; TC adapted ATCC VR-1679
・The concentration of the test virus suspension: 3.5×10∧7 PFU/ml

SpecimenViral infection titer (PFU/cm2) (Note 2)
logarithmic mean
Test results :
Antiviral activity value [R] (Note 3)
1) GlossWell #360 Type Anti-Viral / (2)
Polycarbonate sheets (unprocessed) (Note 1)
Immediately after inoculation
[ Uo ]
5.77
(1) GlossWell #360 Type Anti-Viral / (2)
Polycarbonate sheets (unprocessed) (Note 1)
After leaving it for 24 hours
[ Ut ]
5.41
2) GlossWell #360 Type Anti-Viral /
Polycarbonate sheet (fabricated product)
After leaving it for 24 hours
[ At ]
< 0.80≥4.6
[ Explanation of the values ]
[ Explanation of the values ] : Antiviral activity value ≧4.6: Indicates an antiviral activity value of 99.99% or 1/10000 or more.

( Note 1 ) GlossWell #360 Type Anti-Viral / polycarbonate plate (unprocessed) as a control sample
– control : sample submitted by the client).
( Note 2 ) PFU : plaque forming units
( Note 3 ) Antiviral activity value R = Ut – At

2 ) Host cell verification test
・Test virus: Influenza A virus (H3N2), A/Hong Kong/8/68; TC adapted ATCC VR-1679
・The concentration of the test virus suspension: 4.0×10∧4 PFU/mL

Specimen2)-1 Cytotoxicity2)-2 Confirmation of cell susceptibility to virusesDetermination of test success
Virus infection titer (PFU/mL) (Note 2) Logarithmic mean of normal use
(1) GlossWell #360 Type Anti-Viral /
Polycarbonate sheet (unprocessed)
non-non-establishment
(2) GlossWell #360 Type Anti-Viral / (3)
Polycarbonate sheet (fabricated product)
non-[ St ] 2.48establishment
Negative control (Note 4)non-[ Sn ] 2.60

( Note 4 ) SCDLP medium was used as a negative control.

[ Conditions for acceptance of the study ]
2-1) Cytotoxicity : None
2-2) Confirmation of cell susceptibility to virus : | Sn – Su | ≤ 0.5 and | Sn – St | ≤ 0.5

Testing organization: Microbiological Testing Laboratory, Kobe Testing Center, Japan Textile Quality Technology Center

Antiviral test: feline calicivirus (without envelope)

[ Exam summary ]
・ Test virus: Feline calicvirus; Strain: F-9 ATCC VR-782
・ Host cells: CRFK cells (cat kidney-derived cells)
・ Test sample: ① Paint GlossWell # 360 Type Anti-Viral / ② Glass plate
・ Washing solution: SCDLP medium supplemented with Fetal Bovine Serum to a final concentration of 10%
・ Adhesive film: polyethylene (4cm × 4cm)

Norovirus is rare for cell culture, so it is not possible to test, measure and evaluate the effect of various disinfectants or individual concentrations. For that reason, caliciviruses and mouse norovirus, which are closely related, are our testing, measuring and evaluating the disinfecting effect of general disinfectants.

[ Test method ]
1 ) Main test
1. Prepare the test virus suspension.
2. Place the sterilizing agent filter paper on the bottom of the sterile petri dish, add 4.5 mL of sterile ion-exchanged water, place a U-shaped glass tube so that the test piece does not touch the filter paper for humidity control, and place a processing surface on it. Place the test sample on top.
3. Inoculate 0.4 mL of the test virus suspension into each sample.
4. Cover with the adhesive film and press gently so that the test virus suspension spreads over the entire film.
5. Cover the Petri dish.
6. After leaving at ℃ 25 ℃ for 24 hours put the specimen into the sterilizer stomacher bag, and wash out the virus from the specimen by adding 10mL of washing solution.
7. Measure the virus infectivity by the plaque assay.

2 ) Host cell verification test:
2 ) -1 Cytotoxicity confirmation test
1. Put the sample in a sterilizer stomacher bag, add 10 mL of the washing solution, and perform the washing operation in the same manner as in this test.
2. Incubate at room temperature for 30 minutes.
2. Stain the cells in the same manner as in the plaque assay and check for cytotoxicity.

2 ) -2 Confirmation test of cell susceptibility to -2 virus
1. Put the sample in a sterilizer stomacher bag, add 10 mL of the washing solution, and perform the washing operation in the same manner as in this test.
2. Transfer 5 mL of the above washing solution to a sterilized test tube.
3. Prepare the test virus suspension at 5 x 104 PFU / mL, and add 0.05 mL of the suspension to the washing solution in step 2.
4. Leave at room temperature for 30 minutes.
5. Measure the virus infectivity by the -plaque assay to confirm the sensitivity of the cells to the virus.

[ Test result ]
1 ) Main test
Test virus suspension: Feline calicvirus 1.0 x 107 PFU / mL

SpecimenVirus infection titer (PFU / mL) (Note 2) 
Common logarithmic mean
Glass plate (Note 1)Immediately after inoculation6.47
Glass plate (Note 1)After leaving for 24 hours4.11
GlossWell # 360 Type Anti-Viral painted pieceAfter leaving for 24 hours>2.00
[ Explanation of the values ]
[ Explanation of the values ] : Antiviral activity value >2: Indicates an antiviral activity value of 99% or 1/100 or higher.

2 ) Host cell verification test:

Sample2)-1 
Cytotoxicity
2)-2 
Confirmation of cell susceptibility to virus
Sample2)-1 
Cytotoxicity
Viral infection titer (PFU/mL) (Note 2) Logarithmic mean of normal use
Glass plate (Note 1)None2.44
GlossWell # 360 Type Anti-Viral Paint pieceNone2.41

( Note 1 ) ① A glass plate was used as a control sample.
( Note 2 ) PFU: plaque forming units

2 ) -1
From the results of the cytotoxicity confirmation test, no cytotoxicity was confirmed in any of the samples. In addition, from the results of the test for confirming the sensitivity of the cells to 2) -2 virus, no remarkable decrease in the sensitivity of the cells to the virus was observed in any of the samples.

Testing organization: Microbiological Testing Laboratory, Kobe Testing Center, Japan Textile Quality Technology Center

Antibacterial test / Pseudomonas aeruginosa

[ Test method ]
* Antibacterial test JIS Z 2801 (Film adhesion method)
・ Test strain: Pseudomonas aeruginosa NBRC3080
・ Bacterial solution adjustment solution: 1 / 500NB medium
・ Test bacterial solution inoculation volume: 0.4ml
・ Unprocessed sample: polyethylene film

[ Test results ]

Test sampleNumber of visible bacteria 
Log average
Antibacterial activity value [R] 
(Note 2)
Unprocessed specimen 
(Note 1)
Immediately after inoculation
[U0]      3.87
Unprocessed specimen 
(Note 1)
After culturing for 24 hours[Ut]      5.56
GlossWell # 360 Type Anti-Viral painted pieceAfter culturing for 24 hours[At]      -0.20≧5.8
[ Explanation of the values ]
[ Explanation of the values ] :
Antimicrobial activity value ≧5.8: Indicates an antimicrobial activity value of 99.999% or 1/100000 or higher

( Note 1 ) A polyethylene film was used as a non-processed test piece.
( Note 2 ) Antibacterial activity value R = Ut-At

Testing organization: Microbiological Testing Laboratory, Kobe Testing Center, Japan Textile Quality Technology Center

Antibacterial test / Escherichia coli (O157: H7)

[ Test method ]
* Antibacterial test JIS Z 2801 (Film adhesion method)
-Test strain: Escherichia coli (serotype II O157: H7, verotoxin type I and type II producing strains)
・ Escherichia coil RIMD 0509952
・ Bacterial solution adjustment solution: 1 / 500NB medium
・ Test bacterial solution inoculation volume: 0.4ml
・ Unprocessed sample: polyethylene film

[ Test results ]

Test sampleNumber of viable bacteria 
Log average
Antibacterial activity value【】 
(Note 2)
Unprocessed specimen 
(Note 1)
Immediately after vaccination[U0]      3.89
Unprocessed specimen 
(Note 1)
After culturing for 24 hours[Ut]      4.77
GlossWell # 360 Type Anti-Viral painted pieceAfter culturing for 24 hours[At]    <-0.20≧5.0
[ Explanation of the values ]
[ Explanation of the values ] :
Antimicrobial activity value ≧5.0: Indicates an antimicrobial activity value of 99.999% or 1/100000 or higher.

( Note 1 ) ① A polyethylene film was used as a non-processed test piece.
( Note 2 ) Antibacterial activity value R = Ut-At

Testing organization: Microbiological Testing Laboratory, Kobe Testing Center, Japan Textile Quality Technology Center

Antifungal test / Black mold

[ Test method ]
* Antibacterial test JIS Z 2801 (Film adhesion method)
・ Test strain: Cladosporium cladosporioides NBRC6348 (Black mold)
・ Measurement method: Luminescence measurement method
・ Spore suspension preparation solution: 1/20 SDB medium
・ Spore suspension inoculation volume: 0.4ml
・ Mold spore concentration: 1.0 × 105spores / ml
・ Culture conditions: 25 ° C, 95% RH, 42 hours
・ Unprocessed sample: polyethylene film

[ Test results ]

Test sampleATP amount 
Common logarithmic mean
Growth value 
】(Note 2)
Unprocessed test pieceImmediately after inoculation[Fa] -11.952.4Antifungal activity 
FS】(Note 1)
Unprocessed test pieceAfter 42 hours of culture[Fb]  -9.58
GlossWell # 360 Type Anti-Viral painted pieceImmediately after inoculation[Fo] -13.59≧2.7
[ Explanation of the values ]
GlossWell # 360 Type Anti-Viral painted pieceAfter culturing for 42 hours[Fc] -13.91
[ Explanation of the values ] :
Anti-mold activity value ≧2.7: Anti-mold Indicates that the activity value is greater than 99% or 1/100.

( Note 1 ) Antifungal activity value [FS] = (Fb-Fa) – (Fc-Fo)
( * 2 ) Growth value [F] = Fb-Fa

Testing organization: Microbiological Testing Laboratory, Kobe Testing Center, Japan Textile Quality Technology Center

MOVIE

Emergency : We are very sorry. The explanation is in Japanese only. We hope it will be of some help to you.

Film performance

Test item Test conditionTest result
HardnessUsing Mitsubishi Pencil Uni2H
Adhesion test2 100 squares created, cellophane peel test100/100
Shock resistance testSame as JIS K 5600-5-3 drop test. 300g x 500㎜ (25.4㎜ in diameter)No abnormalities
Acid resistance test% sulfuric acid aqueous solution spot test, 23 ° C x 6 hoursNo abnormalities
Solvent resistanceRubbing test (500g load / 10 reciprocations)No abnormalities
1) No abnormalities in ethanolNo abnormality
2) Toluene is normalNo abnormality
3) Methyl ethyl ketoneNo abnormality
Warm water resistant40 ° C warm water immersion, 100 hoursNo abnormality
Stain resistanceOily magic (black, red) Dry cloth wipeNo abnormality
Contaminated with carbon black, color difference between contaminated and non-contaminated surfaces
⊿E=0.5 less
Weather resistanceSunshine weatherometer (2000 hours)80% gloss retention
Cooling / heat cycle60 ℃ × 3hr ⇔ -20 ℃ × 3hr (10 cycles)No abnormality
Salt spray resistance35 ° C, 5% saline, 500 hoursNo abnormality
Volume resistivityConforms to JIS K6249, Ω ・ cm4.0×10 : 15th power
Dielectric strengthDielectric strength
KV/0.1mm
5.8
Antibacterial and antiviral propertiesSee attached test results
RoHS directive substancesNot contained

Material: Bond steel plate / Film thickness: 6-8μm / Curing condition: After drying at 80 ℃ for 30 minutes, leave at room temperature for 5 days
* The above values are reference values and not standard values.

Precautions for painting

○ Coating environment: Avoid using in an environment with poor ventilation.
○ Pretreatment: Oil, water, and dirt on the surface of the material should be sufficiently removed by solvent degreasing.
○ Painting: Paint immediately. Leaving it for a long time may cause clogging and uneven coating.
・ Manage the film thickness within the specified range.
○ Drying: Organic gas is generated during drying, so provide sufficient ventilation / exhaust.
○ Storage: Store the paint in a cool and dark place.
・ This paint has the property of reacting with moisture in the air. Please seal it after use.
○ Disposal: Follow the MSDS (Material Safety Data Sheet) for disposal of paint residue and waste liquid.
○ Handling Precautions: Do not use in a place with a fire because it uses a flammable organic solvent.
・ Be careful not to come in contact with skin and mucous membranes, especially eyes, as they are irritating.
・ In case of contact, wash with plenty of water.
○ Others: Refer to the product MSDS for details.

Antiviral and antibacterial test results & Documents

SDS Down Load

Recruiting Distributors & Dealers

Our company offers numerous special paints made using cutting edge Japanese chemical technology. Each one of our paints excels at repelling water and oil and withstands submersion. Furthermore, they are also designed to be ultra bright, hard, UV resistant, and heat resistant as well as aesthetically superior. We can offer high added value along with unprecedented new ideas for final surface finishing on all kinds of base materials. We are currently recruiting overseas distributors to carry these superior Japan-made special paints. If you are interested, please contact us through our Contact Form.

Paint in general is treated as hazardous goods, so for shipping charges, we must make individual estimates by airline according to country and region.

First, please send us the Contact Form below. We will calculate the shipping charges to your nearest airport / Term : CIF.
We will then send you a bill including those shipping charges via paypal. After checking that amount, please decide whether or not to purchase our products.
paypal is our only payment method. You can cancel the order at any time.
Furthermore, please note that it will be about 10~14 days before items are shipped.

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    Contact : Recruiting Distributors & Dealers

    BL HY-COATER
    PRESENCE Co., Ltd. / BADLAND
    Post : 230-0073
    2-39-45 Shishigaya Tsurumi-ku Yokohama Kanagawa JAPAN
    TEL +81-45-717-7026 / FAX +81-45-717-7027 / e-mail: info@badland.net

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