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GlossWell #240 Type Anti-Viral / FLOOR

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12/28, 2020 : Inactivation of COVID-19 has been demonstrated by a third-party organization.

December.28,2020 : QTEC (Kobe Testing Center, Japan Textile Products Quality Technology Center) has demonstrated that COVID-19 can be inactivated.
The antiviral and antimicrobial compound (special quaternary ammonium salt) contained in GlossWell #360 Type Anti-Viral has been proven to inactivate COVID-19. The special antiviral and antimicrobial coating of GlossWell #360 Type Anti-Viral has a strong adhesion to the surface on which it is applied, and it also reduces viruses and bacteria on the coating within a short period of time (99.9% reduction after 10 minutes). We hope that COVID-19 will help reduce the risk of contact infections by creating an antiviral environment through coating in areas where many people come into contact with it or where droplets must be avoided.

[ Antiviral test using COVID-19 ]
◯ Client: Presence, Inc.
◯ Testing site: QTEC (Kobe Testing Center, Japan Textile Products Quality Technology Center)
◯ Date of the test result report: December 28, 2020
◯ Test method: ISO 21702
◯ Test virus: SARS-CoV-2 / NIID isolate; JPN/TY/WK-521 (distributed by the National Institute of Infectious Diseases)
◯ Sample : GlossWell #360 Type Anti-Viral ( unprocessed product )
◯ Test sample : GlossWell #360 Type Anti-Viral ( processed product )
◯ Test result: Antiviral activity value after 24 hours of exposure ≧3.2 (▶︎ Numerical explanation)
▶︎ Numerical explanation : Antiviral activity value ≧3.2 means that the antiviral activity value is 99.9% or more than 1/1000.
※ The antiviral activity value that is considered acceptable by ISO 21072 is ≧2.0, so the results of this test far exceed the acceptable value.

Paint manufacturer’s statement
Since GlossWell #240 / #750 Type Anti-Viral has the same composition / component structure as GlossWell #360 Type Anti-Viral and uses the same antiviral and antibacterial compounds (special quaternary ammonium salts), chemical considerations lead us to conclude that it is as effective against COVID-19 as GlossWell #360 Type Anti-Viral.

About GlossWell #240 Type Anti-Viral / FLOOR

GlossWell #240 Type Anti-Viral / FLOOR is a non-slip, pathogenic, anti-bacterial special coating for floors and flat surfaces. Based on an inorganic (real glass) glass coating, it contains special quaternary ammonium salts that inhibit or suppress the growth of viruses, bacteria and fungi, making it a state-of-the-art anti-viral, anti-bacterial and anti-fungal special coating.

It can be applied to flooring, various types of wood, decorative plywood, concrete, marble, tile, and old coatings. It can be used to create an anti-fungal environment in doorways, hallways, tables, and furniture tops. It also has excellent weatherability for outdoor use and chemical resistance to ethanol and other chemicals. We highly recommend this product not only for general social environments where people live, but also for households with pets. [ Made in Japan ]

GlossWell #240 Type Anti-Viral / FLOOR

Inactivate COVID-19.
・2020.12.28 : Inactivation of COVID-19 was demonstrated in an antiviral test conducted by the Virus Research Institute of Japan (QTEC).
・Special quaternary ammonium salts are used.

Anti-viral and anti-bacterial properties.
・Please refer to the list of various test data : 

Inhibits the growth of microorganisms in a short time.
・After 10 minutes, the population of microorganisms in the environment is reduced by 99.9%.

Excellent super water and oil repellent performance.
・It can easily remove fingerprints, various oily substances, blood and body fluids, dirt, etc.

Can be applied by simple painting.
・Cured coating film with excellent adhesion is formed.
・Can be applied to various base materials with only one coat.
・Please check the adhesion in advance.

Excellent in solvent resistance and electrical insulation.
・The coating film is non-flammable.

Transparent and colorless coating after curing.
Forms a smooth final coating with excellent smoothness.

GlossWell #240 Type Anti-Viral / FLOOR provides an “anti-sterilization environment” with a simple application method. A hard anti-sterilization coating with excellent long-lasting effects can be applied to any surface regardless of its shape.

This special functional coating is based on inorganic glass (real glass), which exists in nature. Unlike artificially generated organic glass coatings, it forms a hard anti-bacterial coating with strong adhesion by siloxane bonds to the individual characteristics of various base materials.

Efficacy for COVID-19 : METI of Japan approves

METI of Japan announced
On May 29, 2020, the National Institute of Technology and Evaluation (NITE) and the Ministry of Economy, Trade and Industry (METI) simultaneously announced that quaternary ammonium salts: benzalkonium chloride (already approved) and benzethonium chloride / dialkyldimethylammonium chloride (both added on May 28, 2020) have been added as surfactants effective against a new type of coronavirus. benzethonium chloride/dialkyldimethylammonium chloride (additionally approved on May 28, 2020).

Quaternary ammonium salts approved as effective against COVID-19
◯ Benzalkonium chloride
◯ Benzethonium chloride
◯ Dialkyldimethylammonium chloride

Special quaternary ammonium salt is used as an anti-bacterial ingredient.

The special anti-bacterial compound in GlossWell #360 Type Anti-Viral is a special quaternary ammonium salt based on existing quaternary ammonium salts (benzalkonium chloride / benzethonium chloride / dialkyldimethylammonium chloride).

GlossWell #360 Type Anti-Viral is a special Pathogenic anti-bacterial coating that forms a very slippery film with a low coefficient of friction. Please use GlossWell #240 Type Anti-Viral when applying to floors and corridors.

Construction method

The installation method is extremely simple with one-pack type paint.
・A neutral detergent with excellent oil removal function to remove oil and dirt on the construction surface.
・GlossWell # 360 Type Anti-Viral should be used undiluted without dilution.
・Apply to the construction surface with a brush / brush / microfiber / spray gun / roller / dipping etc.

Estimated range of construction
・GlossWell # 360 Type Anti-Viral x 1ℓ, about 11m2 can be constructed.
・The amount of GlossWell # 360 Type Anti-Viral used for A4 size area should be about 1ml.
・The amount of GlossWell # 360 Type Anti-Viral used when using a non-saturated base / paint brush.
・GlossWell # 360 Type Anti-Viral is completed in a thin film / no thick coating is required.
・The appropriate coating thickness (3 to 8μm) is equivalent to one or two food wrap films.

Apply paint to the construction surface using
・brush, microfiber, spray gun, roller, etc.
・Touch dry time: 30 ~ 40 minutes (You can touch the painted surface with your finger after 30 ~ 40 minutes from painting).
・Drying time at room temperature: 24 hours / However, it takes about 5 days to completely cure the coating.
・In the case of forced drying: 70-80 ° C x 30 minutes / Complete curing is about 3 days at room temperature after forced drying.

Film thickness3~8μm
Application amount50~100g/㎡
Paint viscosity9-10 seconds / HIS NK-2
Recommended spray gun diameter1.3~1.5㎜φ
Spray gun air pressure0.3~0.4MPa(3~4kgf/c㎡)
Touch dry30-40 minutes
Room temperature drying24 hours, full curing about 5 days
Forced drying70-80 ° C x 30 minutes / Complete curing is forcible drying and then drying at room temperature for 5 days

Where the construction should be done

◯ Entrance / corridor / floor / top board of table or desk, etc.
◯ It can be applied to various types of metals, resinous materials, plastic materials, glass, etc.

GlossWell # 240 Type ANTI-VIRAL / FLOOR mechanism

GlossWell # 240 Type ANTI-VIRAL / FLOOR is a one-part special ceramic paint (called binder) that has strong adhesion to various equipment, and special compounds with antiviral / antibacterial functions derived from ammonium are arranged. It has a structure.

GlossWell #240 Type Anti-Viral / Structure of LIGHT

 The following figure illustrates the mechanism by which the special quaternary ammonium salt contained in GlossWell #240 Type Anti-Viral / FLOOR inhibits and suppresses the growth of COVID-19 and other viruses with envelope membranes (such as influenza A viruses). Quaternary ammonium salts, which are also surfactants, disrupt the lipid bilayers and membrane proteins of the envelope of COVID-19. The virus dies when the envelope membrane is destroyed.

GlossWell #240 Type Anti-Viral : A mechanism used to inhibit and suppress the growth of viruses.

Importance of special coatings with antiviral/antibacterial properties

For example, there are many products with antimicrobial function at the manufacturing stage, such as underwear, clothing, bedding, eyeglass frames, train leathers, cutting boards, shoes, etc. However, it is not easy to take antimicrobial measures against “things that are already there, things that exist, and things that cannot be moved. However, when it comes to antibacterial measures against “things that are already there, things that already exist, and things that cannot be moved,” it is not so easy. Although it is possible to “disinfect” such objects with ethanol, how many measures are available in the world to create an “antibacterial environment”?

The answer to this question was to develop a paint with anti-bacterial functions (anti-virus, anti-bacteria, anti-fungus). GlossWell #240 : Type Anti-Viral / FLOOR is a special paint created based on this concept, which provides excellent anti-sterilization performance, super water repellency, and super release performance against oil components. In addition, the entire coating is based on naturally occurring inorganic glass, which provides excellent adhesion (siloxane bonding) to various substrates. After complete curing, the paint film is colorless and transparent. The coating is very smooth to the touch, and there is no sense of discomfort in appearance even if it is applied to existing objects. Of course, anyone can use this product.

About anti-bacterial environment

An antiseptic environment is an environment that inhibits or prevents the growth of pathogenic viruses and bacteria in a variety of environments. In the case of viruses, they can only multiply within the cells they infect, but it has been confirmed that some viruses can survive for a relatively long time in general environments. In particular, preparing an antibacterial environment in advance for objects that are touched by an unspecified number of people and places that are considered to be dangerous is extremely important for preventing contact infection under the current situation of the new coronavirus, which appears to be spreading. In addition, it will become an important social point in the social environment from the viewpoint of public health after this crisis is over.

“Survival time of COVID-19 in the environment “
Airborne (when spread by coughing droplets): 3 hours
Copper surface: 4 hours
Cardboard surface: 24 hours
Plastic surface: 2 to 3 days
Stainless steel surface: 2 to 3 days

[ 2020.3/20 AFP ] : A research team from the Centers for Disease Control and Prevention (CDC), the University of California, Los Angeles, and Princeton University published data in the New England Journal of Medicine.

◯ Rotavirus, the causative agent of infant enteritis, can survive for more than 10 days in the general environment.
◯ The AIDS virus can survive for several days on the surface of various substrates in the general environment.
◯ Influenza viruses can survive for 8 to 48 hours in a general environment. etc.

MOVIE

Emergency : We are very sorry. The explanation is in Japanese only. We hope it will be of some help to you.

Precautions for painting

○ Coating environment: Avoid using in an environment with poor ventilation.
○ Pretreatment: Oil, water, and dirt on the surface of the material should be sufficiently removed by solvent degreasing.
○ Painting: Paint immediately. Leaving it for a long time may cause clogging and uneven coating.
・ Manage the film thickness within the specified range.
○ Drying: Organic gas is generated during drying, so provide sufficient ventilation / exhaust.
○ Storage: Store the paint in a cool and dark place.
・ This paint has the property of reacting with moisture in the air. Please seal it after use.
○ Disposal: Follow the MSDS (Material Safety Data Sheet) for disposal of paint residue and waste liquid.
○ Handling Precautions: Do not use in a place with a fire because it uses a flammable organic solvent.
・ Be careful not to come in contact with skin and mucous membranes, especially eyes, as they are irritating.
・ In case of contact, wash with plenty of water.
○ Others: Refer to the product MSDS for details.

Export of GlossWell Type Anti-Viral from Japan

Please let more people know about this page! 
GlossWell #360 / #240 / #750 Type Anti-Viral is a paint. Since paint is classified as a hazardous material internationally, the cost of exporting it from Japan by air or sea is very high, and it is very difficult for an individual to handle the payment of freight and customs clearance for importation.

In this page, we are announcing a special paint that contains a very effective anti-bacterial compound against COVID-19 : GlossWell #360 / #240 / #750 Type Anti-Viral to corporations, trading companies and government officials in your country. We are disclosing all the necessary evidence to prove the efficacy against COVID-19. All of this evidence has been tested and obtained by public institutions in Japan.

GlossWell #360 / #240 / #750 Type Anti-Viral forms a very tough and special coating with anti-bacterial properties. The coating will adhere to the surface for a long time. If a similar special coating does not exist in your country, please inform any person or entity that can import GlossWell #360 / #240 / #750 Type Anti-Viral or any government official of the existence of this page!

Antiviral test : COVID-19

◯ Sample submission: November 4, 2020 / Response date: December 28, 2020
◯ Client: Presence, Inc.
◯ Test item: Antiviral activity test
◯ Test method: ISO21702 / Measurement of antiviral activity on plastics and other non-porous surfaces
◯ Test organization: QTEC, Japan Textile Products Quality Technology Center, Kobe Testing Center, Microorganism Testing Laboratory

【 Test Summary 】
◯ Test virus: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) / NIID isolate:
JPN/TY!WK-521 (distributed by the National Institute of Infectious Diseases)
・Host cell:VeroE6/TMPRSS2 JCRB1819
・Cytoplasmic drop : Dulbecco’s modified Eagle’s medium (low-glucose) ; DMEM
(SIGMA, Cat#D6046) Minimum Essential Medium Eagle ; EMEM (SIGMA, Cat#-M4655)
・Fetal Bovine Serum : Fetal Bovine Serum (FBS) (SIGMA, Cat#l 73012)
・Sealing film: Polyethylene film
・Control sample : GlossWell #360 Type Anti-Viral (Unprocessed products)
・Test sample : GlossWell #360 Type Anti-Viral (Processed products)
・Purification of test specimen: Not performed
・Inoculation of test virus suspension: 0.4ml
・Test condition: Action temperature: 25°C
・Test condition: Action time: 24 hours (The virus infection titer was also measured immediately after inoculation for the control sample.
・Washing solution: SCDLP diluted 10 times in DMEM containing 2% FBS
・Infection titer measurement method: Plaque measurement method

【 Test Operation 】
1 ) Main Test :
1. Infect host cells with the virus, add EMEM and incubate at 37°C for a predetermined period of time, then centrifuge at 4°C, l,000xg for 15 minutes, and the supernatant is used as the test virus suspension.
2. Dilute the virus suspension obtained in 1. 10-fold with sterile distilled water to 1 ~sx101 PFU/mL, and use this as the test virus suspension.
3. Place each specimen (50 mm x 50 mm) on the bottom of a sterile Petri dish with the processed side up, and inoculate with 0.4 ml of the test virus suspension.
4. Cover the petri dish with an adhesive film (40 mm x 40 mm) and press down lightly so that the test virus suspension is spread over the entire film.
5. Cover the petri dish with the lid.
6. After dying at 25°C for 24 hours at 90%RH or higher, add 10 mL of washing-up liquid to each test sample.
7. Scrub the surface of each test specimen and the dense cling film to wash out the virus.
8. Dilute the washout solution 10-fold using DMEM containing 2% FBS.
9. Determine the viral infection titer by the plaque measurement method.

2 ) Host cell verification test :
2 )-1 Cytotoxicity Confirmation
1. Add l0mL of washing-up liquid to each test sample, and perform the washing-up procedure as in the main test.
2. Make a 10-fold dilution of the washout solution using DMEM containing 2% FBS.
3. Stain the cells as in the plaque measurement method to check for cytotoxicity.

2 )-2 Susceptibility testing of cells to viruses
1. Add 10mL of washing-up liquid to each test sample and perform the washing-up procedure as in the main test.
2. Dilute the washout solution 10-fold using DMEM containing 2% FBS.
3. Take 5 mL of the solution in 2. above into a sterile test tube.
4. Prepare a test virus suspension at 4~6×104 PFU/ml using EMEM, and add 0.05 ml of the suspension to the washout solution in 2.
5. Allow to stand at 25°C for 30 minutes.
6. Measure the virus infection titer by the plaque measurement method and confirm the susceptibility of the cells to the virus by measuring the virus infection titer per ml of washout solution.

Test Results
1 ) Main Test :
・Test virus: SARS-CoV-2 NIID isolate; JPN/TY!WK-521 (distributed by the National Institute of Infectious Diseases)
・Test virus suspension concentration: 1.2 x 107 PFU/ml

 SpecimenVirus infection titer (PFU/cm2) (Note 2)
Logarithmic value for normal use
Virus infection titer (PFU/cm2) (Note 2)
Logarithmic mean value for normal use
Antiviral activity value 【R】
(Note 2)
GlossWell #360 Type Anti-Viral (Unprocessed) (Note 1)
Immediately after inoculation【U0
n1 / 5.525.53
same as aboven2 / 5.525.53
same as aboven3 / 5.555.53
GlossWell #360 Type Anti-Viral (Unprocessed product) (Note 1) After leaving for 24 hours【Utn1 / 5.045.04
same as aboven2 / 5.035.04
same as aboven3 / 5.065.04
GlossWell #360 Type Anti-Viral (Processed product)
After leaving for 24 hours
【At
n1  <1.80 <1.80
same as aboven2  <1.80 <1.80
same as aboven3  <1.80 <1.80 ≧3.2 [ Numeric Description ]

The antiviral activity value ≧3.2 means that the antiviral activity value after 24 hours is 99.9% or more than 1/1000.
※ The antiviral activity value that is considered acceptable by ISO 21072 is ≧2.0, so the results of this test far exceed the acceptable value.

(Note 1) : GlossWell #360 Type Anti-Viral (unprocessed) (provided by the client) was used as a control sample.
(Note 2) PFU : plaque forming units.
(Note 3) Antiviral activity value R= Ut -At

2 ) Host cell verification test
・Test virus: SARS-CoV-2 NIID isolate; JPN/TY/WK-521 (distributed by the National Institute of Infectious Diseases)
・Test virus suspension concentration: 4.9 x 104PFU/ml

Specimen2 ) -1 Presence of cytotoxicity2 ) -2 Confirmation of cell susceptibility to the virus.
Virus infection titer (PFU/ml) (Note 2) Logarithmic mean value for normal use
Determination of test validity
GlossWell #360 Type Anti-Viral (Unprocessed) (Note 1)non-【 Su 】  2.68establishment
GlossWell #360 Type Anti-Viral (Processed product)non-【 Su 】  2.69establishment
Negative control (Note 4)non-【 Sn 】  2.67

(Note 4) A solution of SCDLPs diluted 10-fold in DMEM containing 2% FBS was used as a negative control.

[ Conditions for completion of the test ]
2-1 ) Cytotoxicity: None
2-2 ) Confirmation of cell susceptibility to the virus: | Sn – Su | ≤ 0.5 and | Sn – S1 | ≤ 0.5

【 Reference Information 】
◯ Real-time RT-PCR measurement of virus suspensions subjected to this test.
・Test virus: SARS-CoV-2NIID isolate; JPN/TY/WK.-521 (distributed by the National Institute of Infectious Diseases)
・Virus suspension wastiness: >lOSPFU/ml
・Real-time PCR equipment: Thermal Cycler Dice Real Time SysteM 3 (TaKaRa)
・Detection Kit: SARS-CoV-2Detection Kit -Nl set- (Code NCV-301; Lot# 038200)
(TOYOBO CO.,LTD. Biotech support Department)

◯ Measurement results
The amplification of viral RNA was confirmed by the real-time RT-PCR measurement results (Fig. l).

Fig.I. Real-time RT-PCR measurement results of virus suspension

◯ Graph: red line (102-fold dilution of viral suspension flooding in PBS)
◯ Graph: Blue line (103-fold dilution of virus suspension wastage in PBS)
◯ Graph: black line (104-fold dilution of virus suspension wastage in PBS)
◯ Graph: pink line (Negative control; EMEM)

Testing organization: Microbiological Testing Laboratory, Kobe Testing Center, Japan Textile Quality Technology Center

Antiviral and antibacterial test results & Documents

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Our company offers numerous special paints made using cutting edge Japanese chemical technology. Each one of our paints excels at repelling water and oil and withstands submersion. Furthermore, they are also designed to be ultra bright, hard, UV resistant, and heat resistant as well as aesthetically superior. We can offer high added value along with unprecedented new ideas for final surface finishing on all kinds of base materials. We are currently recruiting overseas distributors to carry these superior Japan-made special paints. If you are interested, please contact us through our Contact Form.

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    BL HY-COATER
    PRESENCE Co., Ltd. / BADLAND
    Post : 230-0073
    2-39-45 Shishigaya Tsurumi-ku Yokohama Kanagawa JAPAN
    TEL +81-45-717-7026 / FAX +81-45-717-7027 / e-mail: info@badland.net

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